Determination of 25 kinds of minerals in Mailuoning Injection by inductively coupled plasma mass spectrometry with microwave digestion / 中草药

ABSTRACT

Objective: To establish an inductively coupled plasma mass spectrometry (ICP-MS) with microwave digestion method for the determination of the amounts of trace elements (Mg, Ca, Fe, Cu, Zn, Mn, Al, B, Ba, Co, Cr, K, Li, Mo, Na, Ni, P, Pb, Sr, Th, Ti, V, As, Cd, and Hg) in Mailuoning Injection. Methods: The conditions of microwave digestion and test were studied respectively. After the microwave digestion, 25 kinds of minerals in the samples were determined by ICP-MS, and the methodology was investigated. Results: The optimal digestion conditions were 3-step slowly heating 400 W 80℃ to heat up for 10 min and keep 5 min; 600 W 120℃ to heat up for 10 min and keep 5 min; 900 W 200℃ to heat up for 20 min and keep 20 min. The determination results of 25 kinds of mineral elements all have a good linear relationship, r≥0.9996. The RSD values of the precision, stability, and repeatability all met the demands of quantitative analysis. The recovery was 94.7%-106.1% and RSD was 0.34%-2.79%. Mg, Ca, Fe, Cu, Zn, Mn, Al, B, Ba, Co, Cr, K, Li, Mo, Na, Ni, P, Pb, Sr, Th, Ti, and V were detected in Mailuoning Injection, and there was no detection of As, Cd, and Hg. Conclusion: This method is simple, sensitive, and precise, and could satisfy the simultaneous determination of minerals in Mailuoning Injection.