Isolation of isoorientin and orientin from bamboo leaf flavones using preparative high-performance liquid chromatography / 中草药

ABSTRACT

Objective: To establish a method for preparation of orientin and isoorientin from bamboo leaf flavones (BLF) using preparative high-performance liquid chromatography (PHPLC). Methods: After BLF was purified by AB-8 resin-based column chromatography, PHPLC was employed to isolate orientin and isoorientin, using a mobile phase consisting of methyl alcohol-water with 0.3% acetic acid (3268). The flow rate was 5 mL/min while the column temperature was maintained at 20℃. The injection volume was 400 μL and the concentration of sample was 15.3 mg/mL. Signal was monitored at 330 nm with the diode array detector. Results: HPLC analysis showed that the purities of isoorientin and orientin were >99% and >93%, respectively, and the recovery rates were (92.7±2.92)% and (85.1±3.45)%, respectively. The confirmation of chemical structures was performed by 1H-NMR and 13C-NMR. Conclusion: The method is simple, with low toxicity but high recovery rate, and can be applied to the separation and preparation of isoorientin and orientin.