Isolation of isoorientin and orientin from bamboo leaf flavones using preparative high-performance liquid chromatography / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2184-2188, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854765
ABSTRACT
Objective:
To establish a method for preparation of orientin and isoorientin from bamboo leaf flavones (BLF) using preparative high-performance liquid chromatography (PHPLC).Methods:
After BLF was purified by AB-8 resin-based column chromatography, PHPLC was employed to isolate orientin and isoorientin, using a mobile phase consisting of methyl alcohol-water with 0.3% acetic acid (3268). The flow rate was 5 mL/min while the column temperature was maintained at 20℃. The injection volume was 400 μL and the concentration of sample was 15.3 mg/mL. Signal was monitored at 330 nm with the diode array detector.Results:
HPLC analysis showed that the purities of isoorientin and orientin were >99% and >93%, respectively, and the recovery rates were (92.7±2.92)% and (85.1±3.45)%, respectively. The confirmation of chemical structures was performed by 1H-NMR and 13C-NMR.Conclusion:
The method is simple, with low toxicity but high recovery rate, and can be applied to the separation and preparation of isoorientin and orientin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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