Influence of different pretreatment methods on stilbenoids in grapevine / 中草药

ABSTRACT

Objective: To study the influence of different pretreatment methods of grapevine on the content of stibenoids in grapevine. Methods: The concentration of resveratrol and ε-viniferin was determined by HPLC, the fresh grapevine sample after picked was pretreated by different methods such as drying in the shade, drying in a dryer under different temperature, keeping in cold storage, slicing, pulverization, or whole treating, and the dynamic changes of stilbenoids in grapevine was investigated. Results: The contents of resveratrol and ε-viniferin in the fresh sample were very low, but the contents of resveratrol and ε-viniferin in the grapevine after picked would change significantly if treated as drying in the shade with whole branch, especially the content of resveratrol would rise from 0.063 mg/g to 7.175 mg/g with the extension of treating time, and the content of ε-viniferin would rise from 0.838 mg/g to 4.225 mg/g, but at some extent, the content would decline. The contents of resveratrol and ε-viniferin would not rise if the sample was pretreated as drying in the shade after slicing, drying in a dryer or keeping in cold storage. Conclusion: The influence of different pretreatment methods to the contents of resveratrol and ε-viniferin is very large, during drying process in the shade with whole branch, the contents of resveratrol and ε-viniferin change obviously and display the tendency of increasing at the earlier phase of the drying process, and then decrease with the extension of time. The contents of resveratrol and ε-viniferin can reach the level of utilization with the pretreatment of drying process in the shade.