Simultaneous determination of six components in Bawei Chenxiang Powder by HPLC-DAD / 中草药

ABSTRACT

Objective: To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid, costunolide, dehvdrocostuslactone, ehydrodiisoeugenol, and acetyl-11-keto-β-boswellic acid in Bawei Chenxiang Powder (BCP). Methods: The chromatographic separation was achieved on a Waters XBridge-C18 column (250 mm × 4.6 mm, 5.0 μm) with methanol-acetonitrile (5050, A) and methanol-0.1% acetic acid (1090, B) as mobile phases at the flow rate of 0.8 mL/min for gradient elution, and the column temperature was 35℃. Its linear relationship, precision, repeatability, stability, and recoveries were investigated. Results: The seven components were well separated and showed good linearity, such as gallic acid 1.5-30.0 mg/L (r = 0.999 0), protocatechuic acid 1.0-20.0 mg/L (r = 0.999 2), costunolide 2.0-40.0 mg/L (r = 0.999 1), dehvdrocostuslactone 2.0-40.0 mg/L (r = 0.999 3), ehydrodiisoeugenol 0.2-4.0 mg/L (r = 0.999 4), and acetyl-11-keto-β-boswellic acid 3.5-70.0 mg/L (r = 0.999 1). The precision was good, and RSD value was less than 2.0%. The repeatability was good, and RSD was less than 2.0%. The stability was good in 24 h. The average recoveries were between 98.49% and 101.55% (RSD ≤ 2.0%). Conclusion: The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of BCP.