Study on removing (-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids by fast centrifugal partition chromatography / 中草药

ABSTRACT

Objective: Using fast centrifugal partition chromatography (FCPC) to remove (-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids. Methods: The technological parameters of orthogonal experiment of FCPC was optimized by flow rate, rotation speed, and loading sample size. The optimized process was compared with the solvent extraction method, and the linear amplification experiments were carried out using the optimal process. Results: The optimized parameters were as follows: rotation speed was 1200 r/min, flow rate was 4 mL/min, and loading sample size was 80 mg/mL. The new prepared alkaloids fraction of Corydalis Decumbentis Rhizoma using amplification experiments has > 94.0% removed rate of BI and >85.0% recovery of the other alkaloids, which were higher than those of the solvent extraction method. This simple method with good repeatability could be completed in a short time. Conclusion: The simple and fast method could improve the safety of Corydalis Decumbens Rhizoma total alkaloids, and provide the industrialization of non-toxic total alkaloids.