Regulation on in vitro myocardial cell L type calcium channel of rats with active component compatibility of Shenfu Decoction / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3594-3598, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854853
ABSTRACT
Objective:
To investigate the regulation of in vitro myocardial cell L type calcium channel of rats with active component of Shenfu Decoction and its combination.Methods:
With Langendorff cardiac infusion, the single ventricular myocardial cell with acute enzymylosis approach was acquired and the voltage current of L type calcium channel was recorded with whole cell patch technique.Results:
The active ginsenosides Re (3, 10, 30, and 100 μmol/L), and Rg1 (10 and 100 μmol/L) can reversibly reduce L type calcium current in a dose dependant manner, Rb1 (10 and 100 μmol/L) has no effect on L type calcium current; the active component aconine (10 and 100 μmol/L) of aconite could irreversibly enhance L type calcium current in a dose dependant manner; The combination of ginsenosides Re (20 μmol/L) and Rg1 (80 μmol/L) also reduced the L type calcium current, which is more significant than either ginsenosides Rg1 and Re; The inhibitory rate of the combination of ginsenosides Rg1, Re and aconine (100 μmol/L) has no difference from the combination of ginsenosides Rg1 and Re.Conclusion:
The active component ginsenosides Rg1 and Re of Shenfu Decoction can reduce the L type calcium current, while aconine can irreversibly enhance the L type calcium current; The combination of ginsenosides Rg1 and Re can obviously increase the reduction of L type calcium current, while the combination of ginsenosides Rg1, Re and anonine can not change the reduction of L type calcium current.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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