Cloning and differential expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in Houttuynia cordata / 中草药

ABSTRACT

Objective: To clone the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) gene from Houttuynia cordata and analyze the differential expression. Methods: The cDNA sequence of DXR was cloned from H. cordata by using RT-PCR strategy. The physical and chemical properties, secondary structure, and three-dimensional structure of the DXR protein were forecasted and analyzed, and its function was predicted. The differential expression of DXR gene in rhizome, stems, leaves, and flowers was analyzed by fluorescent quantitative PCR. Results: The cDNA contained a 1 416 bp open reading frame and encoded a predicted protein of 471 amino acids. Bioinformatics predicted that no transmembrane region and signal peptide were present in DXR. Relative real-time PCR analysis indicated that DXR showed the highest transcript abundance in leaves, moderate level in rhizomes, lower level in stems, and the lowest level in flowers. Conclusion: This study clones DXR gene from H. cordata for the first time, and provides a foundation for exploring the mechanism of this gene for the terpenoid biosynthesis in H. cordata.