Effect of Tianshu Capsule on neurotransmitter in rat model of dura mater following electrical stimulation / 中草药

ABSTRACT

Objective: To study the effect of Tianshu Capsule on the rat dura mater model of superior sagittal sinusin following electrical stimulation and discuss its mechanism. Methods: Male SD rats after brain electrode implantation were started on Tianshu Capsule at the doses of 1.08, 0.36, and 0.18 g/kg. Sumatriptanm Tablets at the dose of 9.72 mg/kg were ig given as a positive drug. Except control and Sham groups, the electrical stimulation was carried out in a voltage of 10 V, frequency of 3 Hz, pulse width of 0.25 ms, and time of 1 h. No operation was taken in the control group. The orbital blood was taken after 0, 30, and 60 min of the stimulation than taking brain tissue. The contents of calcitonin gene-related peptide (CGRP), substance P, and norepinephrine (NE) in plasma and brain tissue were detected by radio immunoassay. Results: Compared with the control group, there was no significant difference of CGRP, substance P, and NE contents in brain tissue of rats in the Sham group. Compared with the Sham group, CGRP in the brain tissue of rats in normal group was increased obviously, while substance P and NE levels were significantly decreased (P < 0.05). Comapred with the model group, the Tianshu Capsule could increase the content of NE in plasma and brain tissue (P < 0.05) while decrease CGRP level (P < 0.05). And Sumatriptanm Tablets could reduce the content of CGRP in plasma (P < 0.05). Conclusion: The Tianshu Capsule could significantly improve the damage of rat dura mater of superior sagittal sinusin following electrical stimulation by releasing neurotransmitter, which may be the mechanisms of migraine treatment.