Genetic diversity of Anoectochilus roxburghii by DALP / 中草药

ABSTRACT

Objective: To investigate the genetic polymorphism of wild Anoectochilus roxburghii and its allied species by molecular marker and find out the genetic differences between them. Methods: Direct amplification of length polymorphism (DALP) was applied to evaluate the genetic variation of 18 populations of A. roxburghii and its allied species from different places. Results: Six pairs of primers were selected to detect 355 polymorphic loci in 18 populations, the percentage of polymorphic bands (PPB) was 100%, and the amplified average polymorphic locus by each pair of primers was 59.2. The percentage of PPB of 14 populations of A. roxburghii was 95.77%, its observed number of alleles (Na) was 1.2734, effective number of alleles (Ne) was 1.074, Nei's gene diversity (H) was 0.0562, Shannon's information index (I) was 0.0969, coefficient of gene differentiation (Gst) was 0.4230, and estimated gene flow (Nm) was 0.6819. Conclusion: The results indicate that A. roxburghii has larger genetic differentiation and lower gene flow among populations. Geographic isolation and wild resource loss may be the major causes of the limited gene flow.