Determination of allantoin in Dioscoreae Rhizoma by LC-MS/MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3627-3629, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854903
ABSTRACT
Objective:
To establish an HPLC-MS/MS method for determination of allantoin in Dioscoreae Rhizoma.Methods:
Determination of LC system used Agilent Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) column and acetonitrile-water (9010) as mobile phase in linear gradient condition. The flow rate was 0.3 mL/min. The mass spectrometer was operated under the negative ion mode with ESI source. MRM (precursor ion of m/z 156.9 and product ion of m/z 96.7) was used to qualify allantoin.Results:
The linear range of the component was 0.12-6.0 μg/mL, r=0.998 5; The average recovery was 101%, and the RSD was 3.2%.Conclusion:
The method is sensitive, reliable, and accurate, and can be applied in the quality control of Dioscoreae Rhizoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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