Isolation and identification of bacteria in fermentation broth of Mirabilitum Praeparatum / 中草药

ABSTRACT

Objective: In order to provide the standardization of production and quality control method of Mirabilitum Praeparatum, the fungi from fermentation broth of Mirabilitum Praeparatum were isolated and identificated, and the fermentation and processing of Mirabilitum Praeparatum were investigated. Methods: The traditional fermentation conditions of Mirabilitum Praeparatum such as temperature and humidity were imitated, using watermelon as culture media. Separation and purification of fungi in fermentation broth of Mirabilitum Praeparatum were carried out on PDA, CZA, and Sabourand media, using plate streaking method. The fungi isolated from fermentation broth of Mirabilitum Praeparatum were identified by morphological observation, microstructure observation, and comparison on DNA ITS sequences. Results: Six fungi were isolated from the fermentation broth of Mirabilitum Praeparatum and identified as WF-1 (Fusarium); WF-2 (Penicillium), WF-3 (Mucor); WF-4, WF-5, and WF-6 (Alternaria). Conclusion: Mirabilitum Praeparatum is produced by salt-tolerant fungi in fermentation broth of watermelon, rather than the traditional view that it is the small crystallization seeping from the watermelon.