Cloning, analysis, and expression of limonoid UDP-glucosyl transferase gene in Citri Reticulatae Semen / 中草药

ABSTRACT

Objective: To cloning limonoid-UDP-glucosyl transferase (LGT) gene in Citrus reticulata, analyze the bioinformations of LGT, and compare the expression to provide the foundation for the composite and regulation mechanism of the active ingredient in Citri Reticulatae Semen. Methods: LGT gene was cloned the protein characteristics were analyzed using bioinformatics and constructing phylogenetic tree. The expression of LGT in the different parts was analyzed using real time-PCR. Results: A 1 530 bp LGT sequence in in C. reticulata, was obtained, which had a 1509 bp ORF and could encode 502 amino acids. And protein analysis and forecast of its secondary and tertiary structures were carried out. Gene expression analysis revealed the expression of citrus LGT was the lowest in semen, and was followed by skin, and the highest expression of fresh. Conclusion: LGT gene of C. reticulata, and is successfully cloned, analyzed, and expressed a basis for the study of matter in the synthesis and regulation of limonoids is provided.