Determination of organic acids and glycosides in different species of Lonicerae Flos / 中草药

ABSTRACT

Objective: To determine and compare the contents of seven kinds of organic acids (neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinicacid, caffeic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, and 4, 5-dicaffeoylquinic acid) and secoxyloganin in Lonicerae Flos. Methods: The contents were determined by HPLC. The analysis was carried out with gradient elution on a Phenomenex C18 colunm eluted with methanol and 0.1% phosphoric acid. The detection wavelength was set at 225 nm. Results: Neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinicacid, caffeic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, and 4, 5-dicaffeoylquinic acid in Lonicerae Flos and secoxyloganin in their linear ranges showed good linear relationship, the average recoveries (n = 9) were 99.41%, 99.05%, 99.01%, 98.71%, 98.79%, 98.82%, 98.89%, and 99.16%, and RSD values were 0.56%, 0.37%, 0.93%, 1.11%, 0.90%, 0.87%, 0.71%, and 1.04%. Conclusion: The method is rapid, simple, accurate, and can be used for quality evaluation of the seven kinds of organic acids in Lonicerae Flos from different germplasm and secoxyloganin.