Research on tissue culture and plant regeneration for roots of Ficus hirta / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2547-2551, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854937
ABSTRACT
Objective:
To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture.Methods:
The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator (6-BA, NAA, 2, 4-D, IAA, KT, and IBA) on the seedling subculture and root culture was studied by orthogonal design.Results:
The best medium for the adventitious bud induction was MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS + 1.0 mg/L 6-BA + 0.3 mg/L IAA + 0.3 mg/L KT; The best rooting medium was 1/2 MS + 1.0 mg/L IBA + 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite (11) matrix, the 30 d survival rate was 93%.Conclusion:
The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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