Studies on anthraquinone biosynthesis in Morinda citrifolia suspension cells / 中草药

ABSTRACT

Objective: To investigate the effects of exogenous hormones, salicylic acid, and methyl jasmonate on suspension cell growth and anthraquinone production of Morinda citrifolia. Methods: After cell suspension lines were established, the cell suspension cultures were treated with exogenous hormones, salicylic acid, and methyl jasmonate, respectively. Then the specific growth rate and anthraquinone production were determined. Results: Exogenous hormone NAA and KT had a significant effect on suspension cell growth and anthraquinone production of M. citrifolia. The optimal medium for the cell suspension culture was B5 + 2 mg/L NAA and 0.1 mg/L KT. The anthraquinone content was up to 588.34 and 595.49 mg/L respectively in the presence of 200 μmol/L salicylic acid and 50 μmol/L methyl jasmonate on day 9, and increased by 17.05% and 18.47% respectively more than those in the control group. Conclusion: The optimum ratio of exogenous hormones combined with exogenous induction of a certain concentration of salicylic acid and methyl jasmonate could promote the production of anthraquinone.