Spectrum-effect relationship of active fraction from Angelicae Sinensis Radix with effect of reinforcing Qi / 中草药

ABSTRACT

Objective: To analyze the correlation between HPLC chromatogram and the effect of different extracted fractions from Angelicae Sinensis Radix (ASR) on reinforcing Qi. Methods: Qi deficiency model of mice was set up by controlling diet and fatigue. The influences of the different extracted fractions from ASR on phagocytotic ability of mononuclear macrophage and immune organs of mice with the symptoms of Qi deficiency were compared, and the active fraction of ASR was screened. The part which was extracted with 70% ethanol (ethanol extract) from ASR has the certain improvement on the Qi deficiency symptoms. The peak areas of each common peak from HPLC fingerprint were correlated with the pharmacodynamic efficacy, and the grey relation statistics was used to study the spectrum-effect relationship. Results: The contribution of various components in ethanol extract with the effect of reinforcing Qi was determined by the spectrum-effect relationship, the order was as follows (characteristic peak number) 18 > 5 > 7 > 12 > 17 > 4 > 19 > 2 > 20 > 6 > 8 > 3 > 14 > 1 > 16 > 11 > 9 > 13 > 15 > 10, peak 7, 10, 11, 13, 17, 18, and 20 are ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide, butylidenephthalide, and levistilide A, respectively. Conclusion: The ethanol extract of ASR has the strong activity of reinforcing Qi, and there is a certain correlation between HPLC specific chromatogram and the effect.