1H-NMR metabonomic study on fecal of chronic unpredicted mild stress model of depression in rats / 中草药

ABSTRACT

Objective: To obtain the potential biomarkers of chronic unpredictable mild stress (CUMS) rats, the change of endogenous metabolites in the faeces of CUMS rats was analyzed using 1H-NMR coupled with metabonomics. Methods: CUMS procedure was conducted for four weeks, CUMS rat model was duplicated, and the faeces of rats was collected at the end of the procedure. The change of endogenous metabolites in faeces was analyzed using 1H-NMR coupled with multivariate statistical analysis. Results: The PLS-DA scores plot demonstrated that behavior indexes of rats in the control group were significantly different from these of rats in CUMS group, suggesting the CUMS model of depression in rats was prepared successfully. Thirty metabolites were identified in the 1H-NMR spectra of faeces, the concentration of glutamine, lactate, and aspartate was increased while that of β-glucose, uracil, tyrosine, and phenylalanine was decreased in CUMS model group with the significant difference compared with the control group (P < 0.05, 0.01). Conclusion: By researching the change of endogenous metabolites in the faeces of CUMS rats, the potential biomarkers in the faeces of CUMS rats are picked up to lay the foundation for the study on the depression pathogenesis and clinical diagnosis.