Effect of Coptidis Rhizoma processed with ginger juice on its property based on metabonomics / 中草药

ABSTRACT

Objective: To explore the difference of hot and cold property of Coptidis Rhizoma (CR) before and after being processed with ginger juice. Methods: To feed rats for 29 d with the decoction of un-processed CR (UPCR) and ginger juice-processed CR (GPCR), the rats' blood was collected at different administration time, the data by HPLC-MS/MS analysis were processed with PCA to determine the metabolites difference between the treatment groups. Results: On the day 29, the metabolic status of endogenous substances in rats' blood showed the biggest difference between the UPCR and GPCR groups. Through checking the endogenous biomarkers of the rats in each group, the content of markers related to amino acid energy metabolism of rats in the GPCR group was higher than that in the UPCR group, indicating the energy metabolism of the GPCR was stronger than that of the UPCR, which showed the differences in cold and hot property between the two drugs. Conclusion: The results show that the differences in potency of the UPCR and GPCR can be reflected by rats' different biological effects. The results are consistent with the traditional processing theory in Chinese materia medica that the cold property of CR could be moderated to be colder by processing with assistant materials with hot property (ginger juice). The work indicates that the metabonomic method is a valuable tool in the research of processing effect on properties of Chinese medicinal substances. The results are helpful to clarify the mechanism of processing CR with ginger juice in the future.