Effect of naringin monomer on osteogenic differentiation of bone mesenchymal stem cells under microstrain environment / 中草药

ABSTRACT

Objective: Under the microstrain environment, to investigate the impact and mechanisms of osteoblast differentiation of the naringin monomer on marrow stem cells (MSCs) in vitro. Methods: The cyclical stretch microstrain was loaded on silicone rubber membrane with cultured rabbit MSCs using EF3200 mechanical tester with the system of BioDynamic biological reaction tank. The experiment was divided into eight groups, A the blank control group in conventional cultured environment; B the group of alone microstrain loading; C the group cultured in environment containing naringin and the naringin concentrations, respectively were 2, 20, and 200 ng/mL; D under microstrain environment, the joint application with naringin at concentration of 2, 20, and 200 ng/mL. Flow cytometry was used to test the proliferation index (PI) of MSCs after microstrain loading. The gene expression of the cells in osteocalcin (OCN), osteoblast specific transcription factor (Runx2) and collagen type I (Col I) were assayed by RT-PCR. Results: Under the microstrain environment combined with naringin, MSCs showed obvious polarity and the long axis of cells parallel to the direction of mechanical stimulus direction. The combination of naringin and microstrain stimulation can significantly improve the MSCs proliferation activity. Naringin (200 ng/mL) could improve the gene expression of OCN under the stimulation of 50000 microstrain. Under 50000 microstrain stimulation, naringin at different concentration could significantly enhance the Runx2 gene expression and the effect between the enhancement and the naringin concentration was positive. Under the microstrain stimulation, naringin at low concentration could promote the Col I gene expression, on the contrary, naringin at high concentration could inhibit the Col I gene expression. Conclusion: Under the microstrain environment, naringin monomer could enhance the proliferation of MSCs and promote the osteogenetic differentiation of MSCs.