Quantitative analysis of three active constituents in crude drug of Cistanche deserticola influenced by different factors / 中草药

ABSTRACT

Objective: To analyze the crude drug of Cistanche deserticola from different sources and to supply some references for the quality control, collection, and processing of C. deserticola. Methods: HPLC-UV method was used to determine the contents of echinacoside and verbascoside, while HPLC-ELSD method was used to determine the content of galactitol. According to the content differences of these compounds, the influences of habitat, processing method, growing period, and space flight on the quality of C. deserticola had been discussed. Results: There were great differences among the samples from various sources. The quality of the crude drugs from the genuine producing areas, such as Inner Mongolia and Xinjiang was better than that from Ningxia. The influences of processing methods were different for the three constituents, the drying method for first killing the enzymes was better to echinacoside and verbascoside, the traditional drying method was better to galactitol. The contents of the active constituents in the crude drug underground > those growing above the ground before florescence > those in florescence > those after florescence. The content of verbascoside was higher in C. deserticola carried by space flight than that not carried by space flight, but the contents of echinacoside and galactitol were almost same. Conclusion: This method supplies a comprehensive analysis of C. deserticola, which could be used for the quality control, collection and processing of C. deserticola.