Gene cloning and sequence analysis of diketide CoA synthase in Amomum villosum / 中草药

ABSTRACT

Objective: To clone the full length cDNA encoding diketide CoA synthase (DCS) gene which plays an important role in curcumin biosynthesis pathway in Amomum villosum, and to provide the basis for the further studies on biosynthesis and gene regulation of curcumin. Methods: According to the annotation of root transcriptome of A. villosum, primers were designed and cDNA of DCS gene was cloned from A. villosum by PCR. Results: The complete coding sequence of DCS gene was 1 170 bp and it encoded a protein of 389 amino acids. The deduced DCS amino acid sequence exhibited 96% identity to the DCS of Curcumae Longae and 66%-69% identity to the chalcone synthase (CHS) of Narcissus tazetta. Phylogenetic analysis on the amino acid sequence of DCS with those of other plants showed that DCS was closely related to Curcumae Longae. Conclusion: The DCS gene is cloned from A. villosum for the first time. This research lays a foundation for studying the gene expression pattern and regulatory functions of DCS in curcumin biosynthesis.