Determination of six triterpene acid contents in effective fraction of Eriobotryae Folium by quantitative analysis of multi-components by single-marker / 中草药

ABSTRACT

Objective: To develop a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of six triterpene acid (euscaphic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid) contents in the effective fraction of Eriobotryae Folium. Methods: Ursolic acid was used as the internal reference substance, and the relative correlation factors (RCF) of euscaphic acid, tormentic acid, maslinic acid, corosolic acid, and oleanolic acid were determined by HPLC-ELSD with good reproducibility. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of effective fraction were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results: For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches. Conclusion: The method established in this research is accurate and feasible that it just needs to assay single-marker (ursolic acid) for the determination of six triterpene acids in the effective fraction of Eriobotryae Folium simultaneously. Therefore, this method could provide a new reference for the quality assess of multi components in Chinese materia medica.