Accumulation of main ginsenoside components in somatic embryogenesis and plantlet regeneration of Panax notoginseng / 中草药

ABSTRACT

Objective: To investigate the accumulation of total saponins and ginsenosides (Rg1, Re, and Rb1) during somatic embryogenesis and plantlet regeneration in Panax notoginseng. Methods: The dynamic accumulation of the total saponins and three components of ginsenosides (Rg1, Re, and Rb1) was analyzed by visible spectrophotometry and high-performance liquid chromatography methods, respectively. Results: The content of total saponins increased from the minimum at embryogenic callus (2.47%) to the maximum at plantlets (7.79%) during somatic embryogenesis development. Because the different specific growth rate of different materials, total saponins accumulation efficiency sort order plantlets > earlier embryos > later embryos > embryogenic callus. We also found that the total content of the three ginsenosides (Rg1, Re, and Rb1) reached the maximum (7.05 mg/g) in the later embryos, and the minimum (2.78 mg/g) was in the embryogenic callus. The contents of ginsenosides Rg1 and Rb1 reached their maximum (2.24 and 4.03 mg/g) respectively in the earlier and later embryos, and the maximum (1.21 mg/g) of ginsenoside Re content was in embryogenic callus. The accumulation (1.947 4 mg/g) of the three ginsenosides minimum occurred in plantlets, and the maximum (5.022 5 mg/g) reached in earlier embryos. Conclusion: Both total saponin contents and accumulation efficiency reach the maximum in plantlets. Three ginsenoside contents and accumulation reach the maximum in later and earlier embryos, respectively.