Cloning and sequence analysis of PcFPS1 gene in Phlegmariurus carinatus / 中草药

ABSTRACT

Objective: To clone and analyze the coding region of Farnesyl diphosphate synthase (FPS) gene PcFPS1 from Phlegmariurus carinatus. Methods: According to the acquired transcriptome dataset of P. carinatus, one transcript coding FPS was obtained. The coding region sequence was obtained using RT-PCR method, and the physicochemical properties, protein secondary structure, and three-dimensional structure of PcFPS1 protein were predictively analyzed. Results: The cloned PcFPS1 gene contained a 1 119-bp coding region for encoding a predicted protein of 372 amino acids with high homology (70%) to FPS gene in Picea abies. PcFPS1 contained almost no transmembrane region and had the conserved domain of terpenoid cylases, without signal peptide. Conclusion: This study clones and analyzes the FPS gene from P. carinatus which is obtained for the first time. The results will provide a foundation for exploring the function of PcFPS1 in terpene and sterol biosynthesis of plants in Huperziaceae.