Optimization of wet ultrafine grinding technology for Hirudo extraction / 中草药

ABSTRACT

Objective: To study the optimal extraction for Hirudo by wet ultrafine grinding technology. Methods: On the basis of single-factor experiments, the response surface analysis was made for exploring the optimal extraction conditions by wet ultrafine grinding technology for Hirudo, with solid-liquid ratio, soaking time, extraction temperature, and grinding time as independent variable and the anticoagulant activity as the response value. According to Box-Behnken principles, the response surface analysis method with four factors and three levels was used. Results: The optimum extraction condition for Hirudo by wet ultrafine grinding technology was as follows: solid-liquid ratio 1:12, soaking time 4.9 h, extraction temperature 6.5°C, grinding time 12 min; The anticoagulant activity could reach 20.13 U/g. Conclusion: The extraction by wet ultrafine grinding technology for Chinese materia medica Hirudo is stable and feasible, and it could be further promoted.