Determination of sodium and potassium in Salviae Miltiorrhizae Radix et Rhizoma and its extract by HPLC-ELSD / 中草药

ABSTRACT

Objective: To establish the HPLC-ELSD method for the determination of sodium (Na) and potassium (K) in Salviae Miltiorrhizae Radix et Rhizoma (SMRR) and its extract. Methods: The column was Waters Sepherisorb SCX column (250 mm × 4.6 mm, 5 μm); The mobile phase was 0.05 mol/L ammomium acetate solution (adjusted to pH 4.8 with acetic acid)-acetonitrile (7030); The flow rate was 1.0 mL/min; The column temperature was 30°C; The drift tube temperature of ELSD was 100°C; The gas flow rate was 2 L/min; And the detector gaining was 2. Results: The calibration curves of Na and K were linear in the ranges of 19.7-197 μg/mL (r = 0.9998) and 35.0-350 μg/mL (r = 0.9995), respectively. The average recoveries of Na and K were 99% (RSD = 0.7%) and 100.2% (RSD = 0.8%), respectively. Conclusion: The method is simple and accurate, and is suitable for determining the contents of Na and K in SMRR and its extract.