Isolation of two benzopyran isoflavones from fruits of Cudrania cochinchinensis and their antitumor activities / 中草药

ABSTRACT

Objective: To study the chemical constituents from the fruits of Cudrania cochinchinensis. Methods: The ethanol extract from the fruits of C. cochinchinensis was separated by silica column chromatography, and the structures were elucidated based on spectroscopy and X-ray diffraction. Results Two known benzopyran isoflavones were isolated and elucidated as 4'-O-methylalpinmumisoflavone (1) and isoderrone (2). The crystal structures showed compounds 1 and 2 belonged to monoclinic system, the space group was P2 (1)/c, and the 3D-supramolecular structures were accumulated by hydrogen bond in intra-and inter-molecule, π-π deposition, and Van der Waals force. The in vitro inhibition on BEL-7404 and SGC-7901 carcinoma cell lines was investigated and the results showed compound 2 had a distinct antiproliferative activity. Conclusion: Compound 1 is isolated from this plant and compound 2 from the plants in this genus for the first time. The plane crystal structures of compounds 1 and 2 are determined by X-ray crystal diffraction, and the 3D stack structures are also reported for the first time.