Protection of peimisine on hepatic fibrosis of rats induced by CCl4 / 中草药

ABSTRACT

Objective: To investigate the protective effect of peimisine on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Methods: The rats were divided into control, model, low-, mid-, and high-dose (2.5, 5, and 10 mg/kg) peimisine groups. Hepatic fibrosis models were induced by ip injection of CCl4 in rats once every 3 d for 8 weeks. The rats in the treatment groups were administered four weeks after the model establishment, once daily until the end of the week 4 after the model establishment. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidasecc (GGT), hyaluronie acid (HA), laminin (LN), type III procollagenc (PC-III), and collegen type IV (IV-C) were assayed, and hepatic tissue contents of hydroxyprolinc (Hyp), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined. The effect of peimisine on hepatic fibrosis in rats was observed. Results: Compared with the model control group, the hepatic fibrosis of rats in peimisine groups was improved obviously, the levels of ALT, AST, ALP, and GGT in serum were lowered obviously (P < 0.05, 0.01), also the serum levels of HA, LN, PC-III, IV-C, and the contents of Hyp and MDA in liver tissue were decreased (P < 0.01), while the level of SOD was increased (P < 0.01). Conclusion: Peimisine has the protective effect on the experimental hepatic fibrosis formation. The possible mechanisms are associated with inhibiting fibrogenesis and fibrosis accumulation, and decreasing lipid peroxidation.