Establishment of fingerprint of active fraction from dried body of Catharsius molossus by capillary electrophoresis and analysis by its total quantum statistical moment / 中草药

ABSTRACT

Objective: To establish capillary electrophoresis (CE) fingerprint of active fraction from the dried body of Catharsius molossus and to explore the feasibility of fingerprint evaluation by total quantum statistical moment method. Methods: Capillary zone electrophoresis (CZE) was used. The electrophoretic conditions were as follows fused silica capillary column (60 cm × 75 μm), borate solution (75 mmol/L, pH 9.8) as the running buffer, applied voltage of +20 kV, and the detection wavelength of 200 nm. Fingerprint was evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method. Results: The CE fingerprint of active fraction from the dried body of C. molossus was established. The similarities in six batches of the active fractions evaluated by similarity analysis software of Pharmacopeia Committee and total quantum statistical moment method had no significant difference. Conclusion: The method is accurate, simple, and reproducible. It could be used for the quality control of active fraction from the dired body of C. molossus. The total quantum statistical moment method could be used to analyze the fingerprint.