Purification technology of total flavonoids in Tricyrtis maculata produced in Shaanxi province / 中草药

ABSTRACT

Objective: To optimize the purification technology of total flavonoids in Tricyrtis maculata (TFTM) produced in Shaanxi province using macroporous adsorption resin. Methods: The content of TFTM was determined with ultraviolet spectrophotometry. Taking the content of total flavonoids purified and transferred by per gram of resin as the indicator, the processes of dynamic adsorption-desorption and static adsorption-desorption were observed, and five macroporous resins, AB-8, D-101, HPD-450, HPD-600, and HPD-700 were optimized. The parallel invastigations were carried out to study the effects of the concentration of extracted liquor, eluent dosage, ratio of diameter to height, and pH value of liquor on the purification technology of macroporous adsorption resin, and to determine the best technological condition. The verification was performed on the optimized extraction conditions. Results: The ratio of diameter to height was 15, the liquid concentration was 1.2 g/mL, the pH value of liquor was 6.47, the raw material to resin was 13, the concentration of eluent was 40%, the sample loading velocity was 1 BV/h, the eluant velocity was 1 BV/h, and the elution volume was 2 BV. Conclusion: The technology has the scientific rationality and could be used to enrich TFTM efffectly.