Genetic diversity analysis on Sophora alopecuroides based on ISSR markers / 中草药

ABSTRACT

Objective: To analyze the genetic polymorphism and relationship of Sophora alopecuroides from Ningxia, Gansu, Qinghai, Xinjiang, and Inner Mongolian in China, and to offer some information for the domestication and protection of wild S. alopecuroides germplasms. Methods: ISSR primers were used to analyze the genetic diversity of 22 populations of S. alopecuroides. Cluster analysis of the unweighted pair-group method with arithmetic average (UPGMA) and principal component analysis (PCA) were carried out based on the molecular data obtained, and a dendrogram was constructed. Results: Totally 433 alleles were amplified using the 51 ISSR primers. The number of allels in per primer range was 5-12, with an average of 8.49. The average percentage of polymorphic loci (PPB) was 93.30%. The Nei's gene diversity (H) and Shannon's information index (I) were 0.3351 and 0.4998, respectively. The genetic distances range was 0.1736-0.6502. Conclusion: The genetic polymorphism among 22 populations of S. alopecuroide is relatively abundant and there are no direct relationship between genetic distance and geographical distribution in them.