Optimization of tissue culture conditions for Pfaffia paniculata / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1334-1337, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-855346
ABSTRACT
Objective:
To establish and optimize the technique of tissue culture and rapid propagation of Pfaffia paniculata.Methods:
Using MS and 1/2MS basic media, the effects of various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seedlings subculture and rooting culture were studied by orthogonal design.Results:
The effective medium for cluster buds-inducing and subculture was MS + 6-BA 1.5 mg/L + NAA 0.1 mg/L + IBA 0.2 mg/L, and the propagation coefficient was over 6.0 per 30 d; The best rooting medium was 1/2 MS + NAA 1.0 mg/L + IBA 0.2 mg/L, and the rooting rate was 100%. The rooting seedlings were transplanted in well-drained sand bed and the survival rate was 98%.Conclusion:
The method in the experiment could provide a basis on protecting the mild resources of P. paniculata exploring the artificial resources, and discussing the new way breedings.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2013
Type:
Article
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