SCoT analysis on genetic diversity among wild populations of Leonurus artemisia in Henan province / 中草药

ABSTRACT

Objective: To investigate the genetic diversity among the wild populations of Leonurus artemisia in Henan province. Methods: The genetic diversity of 66 individuals in eight representational populations of L. artemisia from four areas of Funiu, Taihang, Dabie, and Tongbai Mountains was investigated by SCoT molecular marker technique. Results: A total of 240 bands were produced by 10 primers, among which 238 bands were polymorphic bands, and the percentage of polymorphic bands (PPB) was 99.17%. The average value of Nei's genetic diversity index (H) was 0.264 2, Shannon's information index (I) was 0.39, genetic differentiation coefficient (Gst) was 0.294 6, and the gene flow (Nm) among populations was 1.197.Mantel Test analysis showed that the significant correlation was found between the geographical and genetic distances among the different populations of L. artemisia (r=0.366 3, P < 0.05). Conclusion: The wild populations of L. artemisia in Henan province have higher genetic diversity. There is a significant correlation between geographical and genetic distances.