Genetic diversity analysis on plants in Fritillaria L. from Xinjiang based on ISSR / 中草药

ABSTRACT

Objective: In order to gain genetic polymorphism among different species, the genetic diversity of the plants in Fritillaria L. from Xinjiang was analyzed. Methods: Agarose gel electrophoresis and inter-simple sequence repeat (ISSR) analysis were used to investigate the genetic diversity and genetic relationship of the samples in Fritillaria L. from Xinjiang. Results: Total 185 bands were amplified and detected by 2% agarose gel electrophoresis, including 181 polymorphic bands with the percentage of polymorphic bands (PPB) at 97.84%. Twelve ISSR primers could significantly separate different species of plants in Fritillaria L. from Xinjiang with genetic similarity coefficient (GS) ranged from 0.37 to 0.80 revealed by UPGMA analysis. Ten species in Fritillaria L. from Xinjiang were clustered into four groups using 0.50 as the lowest GS. Conclusion: The results indicate that ISSR technique is useful in revealing the genetic diversity and genetic relationship among 10 species in Fritillaria L., which could provide a scientific basis for the resource collection and identification of the species in Fritillaria L. from Xinjiang.