Effect of Linggui Zhugan Decoction on TNF-α in myocardial tissue and NF-κB and IL-1β in serum of model rats with chronic heart failure / 中草药

ABSTRACT

Objective: To study the mechanism of Linggui Zhugan Decoction (LZD) on chronic heart failure (CHF), through observing the expression of TNF-α and TNF-α mRNA in myocardial tissue and the levels of NF-κB and IL-1β in serum of model rats with CHF. Methods: Model of CHF was produced by ligation of coronary artery. Four weeks after this procedure, rats were randomly classified into model, Captopril (4.375 mg/kg), and low-, mid-, and high-dose (2.1, 4.2, and 8.4 g/kg) of LZD groups. Sham group and the other five groups were ig administered once daily for consecutive 4 weeks. The expression of TNF-α and TNF-α mRNA in myocardial tissue was detected by Western blotting and RT-PCR and the levels of NF-κB and IL-1β in serum were detected by ELISA. Results: The expression of TNF-α and TNF-α mRNA of myocardial tissue was enhanced and the levels of NF-κB and IL-1β in serum of model group were significantly increased compared with Sham group (P < 0.01). The low-, mid-, and high-dose of LZD and Captopril groups could inhibit the expression of TNF-α and TNF-α mRNA in myocardial tissue and decrease the levels of NF-κB and IL-1β in serum significantly compared with the model group (P < 0.05, 0.01). Conclusion: The mechanism of LZD interfering CHF seems to be related to the regulation of the cytokine network inflammatory factors.