Genetic diversity of Artemisia annua in natural populations of China revealed by SRAP marker / 中草药

ABSTRACT

Objective: The genetic diversity of Artemisia annua germplasmic resources in natural populations of China was analyzed by SRAP marker. Methods: Taking 60 germplasm of A. annua version in main distribution areas of natural population in China as testing materials, the relative parameters were calculated by Popgene version 1.31 and Treeconw software. The systematic diagram of genetic relationship was made up by Treeconw software and clustered by UPGMA method. Results: The 232 DNA bands were amplified with 24 pairs of SRAP primer combinations, and the percentage of polymorphic bands (PPB) was 81.47%. At species level Nei's gene diversity (H) was 0.190 5 using SRAP marker. Shannon's information index (I) was 0.300 0 by SRAPs; Among the four areas with higher level of artemisinin PPB were 50.00%-61.21%, the average was 55.82%. He were 0.155 7-0.179 3 by SRAP marker. I were 0.237 9-0.276 6 by SRAP maker; Genetic distance were 0.643 2-0.872 7, the average was 0.754 7. Conclusion: SRAP marker is an efficient method in revealing the abundant genetic diversity of wild A. annua in China, which provides the material basis for promising germplasm of further breeding.