Analysis of alkaloids in Nelumbo nucifera leaves by solid phase extraction-rapid resolution liquid chromatography-quadrupole-time of flight mass spectrometry / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1066-1068, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-855574
ABSTRACT
Objective:
A novel method for the analysis of alkaloids in Nelumbo nucifera leaves was established by SPE-RRLC-Q-TOF mass spectrometry.Methods:
The crude sample was extracted by 1% HCl with ultrasound-assisted extraction, then purified by SPE column, and eluted with ammonia-methanol (595). After concentration, the residue was dissolved by methanol solution. The real sample was analyzed by RRLC-Q-TOF. A Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water as mobile phase. The elutes were detected by Q-TOF to obtain the MS spectra with extract molecular weights under positive ion mode.Results:
Nine alkaloids were identified.Conclusion:
This method can be used to rapidly determine the alkaloids of N. nucifera leaves.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2011
Type:
Article
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