Quantitative analysis of multi-components with single marker for determination of alkaloids in Phellodendri amurensis cortex / 中草药

ABSTRACT

Objective: To establish an HPLC method for simultaneously measuring the content of berberine, palmatine, and jatrorrhizine in Phellodendri Amurensis Cortex, and on this basis, the multiple components were examined by way of using a single test to validate the method's feasibility and technical suitability of quality control applications for Phellodendri Amurensis Cortex. Methods: Taking berberine, the main component in Phellodendri Amurensis Cortex, as an indicator, a relative correction factor between berberine and palmatine as well as jatrorrhizine was established. Then the correction factor was used to calculate the content of the other two in Phellodendri Amurensis Cortex. In this way, the calculated value was compared with the external standard method. Results: A measuring method of evaluating the three alkaloids in Phellodendri Amurensis Cortex was established via using a single test; There was no significant difference compared with the external standard method. Conclusion: The method for simultaneously measuring the content of berberine, palmatine, and jatrorrhizine in Phellodendri Amurensis Cortex is reliable and accurate, it could be used to control the quality of crude drugs and herbal pieces of Phellodendri Amurensis Cortex.