Absorption mechamism of dehydroandrographolide in human caco-2 cell monolayer model / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1154-1157, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-855583
ABSTRACT
Objective:
To study the absorption mechanism of dehydroandrographolide (DAL) in human Caco-2 cell monolayer model.Methods:
Caco-2 cell monolayer model was applied to investigate the bidirectional transport of DAL. The effects of time, drug concentration, temperature, and P-gp inhibitor (Verapamil) on the absorption of DAL were observed. Drug concentration was measured by LC/MS/MS and the apparent permeability coefficients (Papp) were calculated.Results:
With the time and concentration increasing, the bidirection transport of DAL in Caco-2 cell monolayer model was of time and concentration dependence, without saturation. And it was not influenced by the change of temperature and the presence of Verapamil.Conclusion:
The absorption and transport of DAL are passive diffusion as the dominating process in Caco-2 cell monolayer model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2011
Type:
Article
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