Multicolor fluorescence in situ hybridization analysis on rDNA and telomere of Isatis indigotica / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 972-975, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-855610
ABSTRACT
Objective:
To investigate the physical location of 45S, 5S rDNA, and telomere sequences and analyze the karyotype of the metaphase chromosomes of Isatis indigotica.Methods:
Multicolor fluorescence in situ hybridization (FISH) approach was used to detect the physical location of the above sequences.Results:
One pair of 45S rDNA sites located on chromosome 4, one pair of 5S rDNA sites located on chromosome 5 and 14 pairs of telomere sites located at the ends of each chromosome in I. indigotica were detected. The karyotype formula is 2n = 2x = 14 = 10m (2SAT) + 4sm, which belongs to 2A karyotype.Conclusion:
This research provides effective cytological markers for karyotype analysis and construction of a molecular cytogenetic map of I. indigotica.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2011
Type:
Article
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