Effect of Haobie Yangyin Ruanjian Decoction on rats with hepatic fibrosis induced by CCl4 composited factor / 中草药

ABSTRACT

Objective: To observe the effect of Haobie Yangyin Ruanjian Decoction (HYRD) on rats with hepatic fibrosis induced by CCl4 composited factor. Methods: Wistar rats were randomly divided into seven groups control group, model group, HYRD groups of low, medium, and high doses, positive groups of Fufang Biejia Rungan Tablets, and colchicin. Model of hepatic fibrosis was induced by CCl4 composited factor. Malondialdehyde (MDA), total protein (TP), albumin (ALB) in serum and hydroxyproline (Hyp) in liver were detected by chromatometry; MMP-2 and MMP-9 activities in liver were detected by gelatin zymography; α-SMA expression in liver was evaluated by immunohistochemical method. Results: MDA in serum and Hyp in liver were decreased gradually in HYRD groups compared with those of model group; TP and ALB in serum were increased gradually in HYRD groups compared with those of model group; MMP-2 activity in liver was decreased in HYRD groups compared with those of model group; MMP9 activity had no significant change in each group and α-SMA expression was decreased gradually in HYRD groups compared with those of model group. Conclusion: HYRD can antagonize the hepatic fibrosis induced by CCl4 composited factor.