Genetic diversity in cultivated populations of Dendrobium officinale / 中草药

ABSTRACT

Objective: To investigate the genetic diversity in cultivated populations of Dendrobium officinale, providing the basis for the protection of germplasm resources and breeding of new varieties. Methods: A molecular marker RAPD was used to analyze the genetic diversity of 14 populations of D. officinale and a population of D. devoninum from Yiwu, Tiantai, Jiande, Wuyi in Zhejiang Provinces and Malipo, Menghai in Yunnan Provinces. Results: Fifteen primers screened from 225 RAPD primers were used in the PCR amplification of 105 samples of D. officinale and 3 samples of D. devoninum; And 138 bands were generated among which 133 bands were polymorphic. The percentage of polymorphic bands (PPB) was 96.38%; The percentage of polymorphic loci (PPL) for 14 populations of D. officinale ranged from 10.79% to 76.26% was 45.32% (mean). Conclusion: Cultivated D. officinale of main producing places in whole country is rich in genetic diversity. Genetic relationship of D. officinale has a obvious correlation with its geography provenance, but nothing to do with the cultivation place.