Determination of five flavonoids in Cacumen Platycladi Carbonisatum by UPLC / 中草药

ABSTRACT

Objective: To establish a UPLC method for simultaneous determination of five flavonoids (myricetin, quercitrin, quercetin, kaempferol, and amentoflavone) in Cacumen Platycladi Carbonisatum. Methods: The analysis was performed on a Waters Acquity UPLC system with a Waters BEH C18 column (50 mmx2.1 mm, 1.7 mm). The five flavonoids were separated with gradient mobile phase consisting of 0.05% aqueous formic acid and methanol. The temperature of column was 30°C, and the injection volume was 2 μl/min, detection wavelength was 360 nm. Results: The five flavonoids including myricetin, quercitrin, quercetin, kaempferol, and amentoflavone had good linearity (r≥0.999 5) within the linear ranges. The average recovery rate was 96.85% - 99.01% with RSD ≤4.00%. Conclusion: The developed UPLC method is simple, sensitive and accurate and has the good repeatability in separation, which is available for the quality control of Cacumen Platycladi Carbonisatum.