Analysis on three isozymes in plants of Curcuma L / 中草药

ABSTRACT

Objective: To explore the interspecific and intraspecific genetic relationships of six species in Curcuma L. Methods: The superoxide dismutase (SOD), polyphenol oxidase (PPO), and cytochrome-oxidase (COD) were studied by vertical slab Polyacrylamide gel electrophoresis (PAGE). The three isozymes data were analysed by using clustering method UPGMA. Results: The six species in Curcuma L. can largely be distinguished by zymogram of three isozymes; Moreover, PPO and COD can be used to distinguish the different accessions in the same species of Curcuma L. Conclusion: The genetic relationships among different accessions are related with the geographical origin. Clustering approach could make a distinction between Curcuma longa and C. sichuanensis. The differentiation has happened between the wild and cultivated species of Curcuma L.