Effect of Polyporus umbellatus polysaccharides on activation of murine bone marrow dendritic cells via Toll-like receptor 4 / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 118-123, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-855708
ABSTRACT
Objective:
To explore the mechanism of immunomodulatory activity of Polyporus umbellatus polysaccharides (PPS) on murine bone marrow dendritic cells (BMDC).Methods:
BMDC phenotype and the function indexes were observed by 3H-TdR incorporation, ELISA, and flow cytometry.Results:
Compared with the negative group, PPS could increase the co-expression of CD11c and CD86 molecules on dendritic cells (DC) surface and the production of IL-12 and IL-10 in a dose-dependent manner. PPS also enhanced matured BMDC capacity of T cell initial activation and decreased phagocytosis of BMDC. Anti-Toll-like receptor 4 (TLR4), but not anti-TLR2 or complement receptor 3 (CR3) monoclonal antibodies inhibited PPS-induced production of IL-12 p40 and blocked the combination between fluorescence-labeled PPS (f-PPS) and BMDC.Conclusion:
The data demonstrate that PPS could promote the activation of murine BMDC via TLR4 and maturation of immunomodulationy activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2011
Type:
Article
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