Analysis of genetic relationships among species of Lycoris Herb. by AFLP markers / 中草药

ABSTRACT

Objective: To investigate the interspecific relationships among thirteen species (include two varieties) of Lycoris Herb, from different habitats and provide the evidence for evaluation and exploitation of the germplasm resources for Lycoris Herb. Methods: The genetic diversities of 20 accessions of Lycoris Herb, from different habitats were investigated with the technique of amplified fragment length polymorphism (AFLP). Results: Six pairs of AFLP primers were selected from 33 pairs of combinations, and a total of 330 amplified clear DNA bands were obtained, among them 320 were polymorphic bands. The percentage of polymorphic bands was 96.8%. The coefficient ranges of interspecific genetic similarity (GS) in AFLP analyses for the 20 materials were 0.38-0.89. AFLP clustering results showed that the materials from the same habitats had a closer genetic relationship. There were abundant genetic diversity in species of Lycoris Herb. Conclusion: AFLP technique is efficient in generating more accurate information on the genetic diversity and relationship in plants of Lycoris Herb.