Effect of different metal ions for submerged culture of Shiraia bambusicola / 中草药

ABSTRACT

Objective: To study the effect of different levels of Cu2+, Fe3+, Cr3+, and Ca2+ on mycelia biomass and the hypocrellin yield by submerged culture of Shiraia bambusicola. Methods: The fungis were cultured under 27°C and 130 r/min for 96 h with each level of metal ions. Results: The biomass and the hypocrellin yield were the highest with Cr3+ of 0.005%; Fe3+ of 0.003% and 0.005% contents were favorable to the biomass and the hypocrellin yield, respectively; Ca 2+ content of 0.03% was suitable for the mycelia ' growth and the hypocrellin yield benefited from the concentration of 0.005%; Cu2+ content of 0.03% was added, both the biomass and the hypocrellin yield reached the best. Conclusion: The highest mycelia dry weight and the hypocrellin yield could reach 15.28 g/L and 8.12 mg/g, respectively in addition of metal ion.