Effects of triptolide on cell cycle and expression of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1819-1823, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-855747
ABSTRACT
Objective:
To investigate the effect of triptolide on cell proliferation, cell cycle distribution, DNA and protein expression regulation of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells.Methods:
Cell viability was detected by MTT assay and cell cycle distribution was measured by flow cytometry. Effect on mRNA expression of P21wap1/cip1 and P27kip1 was detected by RT-PCR. The change of protein expression was measured by Western blotting.Results:
Triptolide of varying concentration significantly induced the inhibition of proliferation in a dose-dependent manner and G0/G 1 phase arrest of the cell cycle progression. The events were coincided with the upregulation of the mRNA and protein expression of P21wap1/cip1 and P27kip1.Conclusion:
These results suggest that triptolide might exhibit its strong antitumor effect via alternation of P21wap1/cip1 and P27kip1. It provides framework for a clinical evaluation of triptolide.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2010
Type:
Article
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