Effects of triptolide on cell cycle and expression of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells / 中草药
Chinese Traditional and Herbal Drugs
; (24): 1819-1823, 2010.
Article
in Zh
| WPRIM
| ID: wpr-855747
Responsible library:
WPRO
ABSTRACT
Objective: To investigate the effect of triptolide on cell proliferation, cell cycle distribution, DNA and protein expression regulation of P21wap1/cip1 and P27kip1 in human multiple myeloma RPMI 8226 cells. Methods: Cell viability was detected by MTT assay and cell cycle distribution was measured by flow cytometry. Effect on mRNA expression of P21wap1/cip1 and P27kip1 was detected by RT-PCR. The change of protein expression was measured by Western blotting. Results: Triptolide of varying concentration significantly induced the inhibition of proliferation in a dose-dependent manner and G0/G 1 phase arrest of the cell cycle progression. The events were coincided with the upregulation of the mRNA and protein expression of P21wap1/cip1 and P27kip1. Conclusion: These results suggest that triptolide might exhibit its strong antitumor effect via alternation of P21wap1/cip1 and P27kip1. It provides framework for a clinical evaluation of triptolide.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional and Herbal Drugs
Year:
2010
Type:
Article