Preliminary study on Anemarrhena asphodeloides tissue culture / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1886-1889, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-855755
ABSTRACT
Objective:
The tissue culture of Anemarrhena asphodeloides was preliminarily studied to establish A. asphodeloides regeneration system.Methods:
The establishment of A. asphodeloieds sterile system, tiller bud proliferation, tiller callus induction and its re-differentiation as well as transplanting of regenerated plantlets were studied by plant tissue culture and single factor test method.Results:
The best disinfection way of A. asphodeloides seeds was firstly dealt with 75% ethanol for 30 s and then dealt with 0. 1% HgCl2 for 15 min; The best medium of bud proliferation for A. asphodeloides tillers was MS+KT 1 mg/L+NAA 0.5 mg/L; The best medium of A. asphodeloides tiller callus induction was MS+KT 2 mg/L+NAA 0.5 mg/L; The best medium of A. asphodeloides tillers callus redifferentiation was MS+ KT 2 mg/L+NAA 0.1 mg/L; The best rooting medium of A. asphodeloides callus regeneration buds was 1/2 MS+NAA 0.5 mg/L; The best transplanting substrate of A. asphodeloides plantlets was humus soil.Conclusion:
The regeneration system of A. asphodeloides is established, which provides a technological basis for factory production of A. asphodeloides plantlets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2010
Type:
Article
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