Effects of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism / 中国临床药理学与治疗学

ABSTRACT

AIM: To explore the effect of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism. METHODS: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression level of linc00467 in the plasma of LAD patients and healthy volunteers. The overall survival (OS) was analyzed by Kaplan-Meier survival analysis and log-rank tests. Cell proliferation assays and Xenograft mouse model were used to confirm the effect of linc00467 expression on tumorigenesis in vitro and in vivo.RESULTS: linc00467 expression was up-regulated in the plasma of LAD patients compared with healthy volunteers. In addition, high levels of linc00467 expression were correlated with larger tumour sizes, lymph node metastasis and advanced TNM stages. High levels of linc00467 indicated a poor prognosis in LAD patients, multivariate analyses indicated that linc00467 expression could serve as an independent prognostic factor for overall survival of LAD. Functional experiments showed that knockdown of linc00467 could inhibit LAD cell proliferation in vitro and in vivo. CONCLUSION: linc00467 is involved in the progression of LAD and that linc00467 may be a novel diagnosis biomarker and a potential therapeutic target for LAD.